03UV Extracorporeal Blood Ozone and Oxygenation (EBOO) + UV Light: 

A Leading-Edge Therapy for Optimal Wellness

03UV Extracorporeal Blood Ozone and Oxygenation (EBOO) + UV Light is a leading-edge therapy with a wide range of whole-body benefits and the combination of ozone with UV light is an innovative therapeutic modality. It is an excellent stand-alone therapy and also compliments a wide range of treatments to optimize the process of achieving sustained wellness. 

In this blog you will learn:

  • What EBOO and 03UV are.
  • What ozone is.
  • What ozone therapy is.
  • What ultraviolet (UV) light is.
  • What ultraviolet light therapy is.
  • How to utilize EBOO and 03UV in your path to optimal wellness.
  • What it is like to receive EBOO/03/UV therapy.

Ozone

Ozone (O3) is a pale blue gas with a distinctive smell (like after a thunderstorm) that was discovered in the mid-nineteenth century. It is a molecule consisting of three atoms of oxygen in a dynamically unstable structure. At high altitudes, ozone makes up a significant portion of the Earth’s ozone layer that protects the planet from the sun’s ultraviolet rays.

Ozone and Air Pollution

Regrettably, for most individuals, the term “ozone” tends to be associated solely with pollution. There’s a widespread misconception that ozone is the primary component of pollution. However, when combustion engines and factories began emitting significant quantities of hydrocarbons, these molecules, due to their reactive nature, began reacting with oxygen in the presence of water vapor, resulting in oxidized hydrocarbons known as peroxides. Occasionally, this reaction generates ozone, which then rapidly reacts with another hydrocarbon molecule to produce more peroxides. Moreover, the involvement of atmospheric nitrogen leads to the formation of another group of compounds called nitrates. Collectively, the combination of atmospheric water vapor (humidity), hydrocarbons, peroxidized hydrocarbons, nitrates, and ozone forms a rather noxious mixture known as smog. 

Scientists needed a method to quantify the extent of smog, so they turned to measuring ozone, and it wasn’t because it was the most irritating of substances, but rather for three pragmatic reasons:

  1. Ozone levels in the air closely parallel the level of pollution present
  2. Ozone is simple and cost-effective to measure accurately
  3. Measuring the primary constituents of smog is exceedingly intricate and expensive 

Thus, when ozone levels soar in urban areas, it indicates a corresponding rise in peroxides and nitrates. Excessive ozone levels prompt warnings to individuals with respiratory ailments to stay indoors. However, these warnings aren’t issued due to the potential respiratory problems posed by ozone itself. While ozone is indeed a potent respiratory toxin when inhaled directly, its levels compared to peroxides and nitrates are negligible. Even under the most severe pollution conditions, ozone concentrations aren’t high enough to induce respiratory symptoms. The alerts serve to caution people about the presence of significant amounts of nitrates and peroxides, which can cause substantial respiratory harm. 

Nevertheless, because ozone monitoring has been utilized for the past three decades to gauge pollution levels, and owing to ozone’s status as a potent respiratory toxin, it has erroneously been perceived as the primary irritant in pollution.

Ozone and Environmental Sterilization

Ozone does have practical environmental uses due to its strong oxidizing capacity. It has been proven to be highly effective in killing bacteria, fungi, and molds and inactivating viruses both on the surfaces and in the air. 

Uses include disinfection of: 

  • Air. 
  • Water.
  • Surfaces.
  • Foods.

In addition to external uses of ozone, there is significant evidence suggesting that ozone has a broad range of applications when applied to the body through routes aside from inhalation.

Ozone can be applied in a multitude of ways to the human body and can be insufflated rectally, vaginally, into the bladder as well as into the sinuses and ears.  There are dental applications for ozone and ozone can be injected into muscles, scar tissue, tendons, joints, and ligaments. Water can be ozonated and drunk, olive oil can be ozonated and applied topically to the skin.  Limbs can be bagged (especially if there are infections, poorly healing wounds, or gangrene). One can breathe ozone but it has to be bubbled through olive oil first otherwise it can damage the alveolar lining. This article will focus on blood ozonation in particular.

Blood Ozonation

Blood ozonation was discovered by Dr. Hans Woolf in 1961 and Nikola Tesla patented the first ozone generator in 1896. As early as 1911 ozone was already being used as a treatment for many diseases such as tuberculosis, anemia, whooping cough, tinnitus, respiratory disease, insomnia, diabetes, gout, and syphilis.


EBOO (Extracorporeal Blood Ozone and Oxygenation) is an advanced procedure that removes around 2 liters of a person’s blood, treats it with a mixture of ozone and oxygen, and returns it to the body, delivering up to 50% of total blood volume during a session. This procedure, which is also known as ozone dialysis, uses a dialysis membrane that allows blood to flow against a countercurrent of ozone gas (1). This is a significant evolution in relation to prior forms of ozone therapy in terms of ozone application and ease of the procedure.

Benefits of Blood Ozonation

Optimization

Ozone therapy is a potent therapy for those looking to optimize wellness. 

Potential benefits include:

  • Healthy circulation.
  • A balanced inflammatory response.
  • Enhanced immunity.
  • Increased nitric oxide levels.
  • Improved wound healing.
  • Reduced risk of infection.
  • A healthy pain response.
  • Reduced swelling.
  • Reduced bruising.
  • Improved cognitive function.
  • Increased energy.

Healthspan

EBOO may also have healthspan benefits including:

  • Reduced oxidative stress.
  • Increased levels of antioxidants.
  • Improved mitochondrial function.

As per a 2022 study:

… the bioenergetic health index in PBMC (peripheral blood mononuclear cells) improves significantly after just 2 OHT (ozone high dose therapy) applications over a period of 1 week. The overall improvement of the BHI (bioenergetic health index) is based primarily on a significant increase in the reserve capacity and the maximum respiration of the mitochondria (2).

The bioenergetic health index is a unique way to measure mitochondrial health through sophisticated measurements of circulating leukocytes and platelets, which act as a marker for physiological mitochondrial function. It is promising to see that ozone therapy may improve this parameter as mitochondrial health is a crucial component of both optimal wellness and the healing process.

Acute and Chronic Infections

Evidence suggests a significant impact of ozone therapy in a range of infectious diseases, including bacterial, viral, and fungal through both direct oxidation of the microorganism and stimulation of the immune system. 

Case studies indicate that difficult-to-treat infections such as Ebola and Chronic Lyme B disease may be an effective target for ozone therapies (3).

Athletic Performance

There is some evidence that EBOO may also have potential benefits for athletic performance. In one study, athletes who were treated with EBOO showed significant improvements in their V02 max after ozone therapy twice weekly for five weeks (4), a measure of aerobic fitness.

Although there is an impression that blood ozonation functions primarily through direct action of ozone on pathogens, the reality of the mechanisms through which the procedure functions are more intricate and complex.

Conditions Supported by EBOO

Although EBOO doesn’t cure any disease, it may be supportive in the treatment of certain health conditions. 

These conditions may include:

  • ​​Infectious disease
  • Autoimmune disease
  • Chronic fatigue syndrome
  • Peripheral arterial disease
  • Coronary disease 
  • Raynaud’s disease
  • Cholesterol embolism
  • Severe dyslipidemia
  • Chronic fatigue syndrome
  • Lyme disease
  • Multiple sclerosis
  • Rheumatoid arthritis
  • Cancer
  • HIV/AIDS
  • Heart disease
  • Stroke
  • Alzheimer’s disease
  • Parkinson’s disease
  • Neurodegenerative diseases in general

Ozone: Mechanisms of Action

Medical ozone used in the O2-O3 therapy has nothing to be shared with the activity of ozone against microbes, as in the first case ozone exerts its action indirectly via the hormetic-mediated activity of molecules such as lipid ozonation products (LOPs) (5). According to research, there is no direct effect of ozone on microbes in the blood.

Although ozone’s mechanism of action once it enters the body isn’t through the direct activity of ozone on microbes, there is a direct impact of ozone’s oxidative properties on microbes when it travels through the ozone filtration system outside of the body. 

Whereas in-tact ozone doesn’t enter the body directly, ozone metabolites do. These include ozonides and lipid ozonation products (LOPs) and are responsible for many of the beneficial impacts of ozone therapy.  

The therapeutic efficacy of ozone therapy may be partly due to the controlled and moderate oxidative stress produced by the reactions of ozone with several biological components (6) including intracellular and mitochondrial components (hormesis and mitohormesis respectively).

Hormesis: Beneficial Stress

A hormetic mechanism is central to the therapeutic aspects provided by ozone therapy. Hormesis is a term used by toxicologists to refer to a biphasic dose response to an environmental agent characterized by a low dose stimulation or beneficial effect and a high dose inhibitory or toxic effect (7). In the fields of biology and medicine hormesis is defined as an adaptive response of cells and organisms to a moderate (usually intermittent) stress (8).

The hormetic pathway includes hormetic factors, cell stress and signaling, and hormetic effectors.

The Hormetic Cascade with Examples

Ozone therapy induces a state of moderate hormetic oxidative stress. This activates nuclear transcriptional factor, nuclear factor-erythroid 2-related factor 2 (Nrf2). Nrf2 then induces the transcription of antioxidant response elements (ARE) (9). Transcription of ARE results in the production of numerous antioxidant enzymes, such as SOD, GPx, glutathione-s-transferase(GSTr), catalase (CAT), heme-oxygenase-1 (HO-1), NADPH-quinone-oxidoreductase (NQO-1), phase II enzymes of drug metabolism and heat shock proteins (HSP) (10).

Studies have demonstrated that Nrf2 contributes to the anti-inflammatory process by orchestrating the recruitment of inflammatory cells and regulating gene expression through the antioxidant response element (ARE) (11). The Keap1 (Kelch-like ECH-associated protein)/Nrf2 (NF-E2 p45-related factor 2)/ARE signaling pathway mainly regulates anti-inflammatory gene expression and inhibits the progression of inflammation (12).

This inhibition of the inflammatory cascade is a crucial component of slowing or stopping the progression of various chronic health conditions including biotoxin/environmentally induced chronic inflammatory response syndrome (CIRS). By reducing the vicious cycle of uncontrolled inflammation, the body is able to start shifting out of an emergency state of reactivity and towards a state of healing.

In addition, Nrf2 controls the basal and induced expression of an array of antioxidant response element–dependent genes to regulate the physiological and pathophysiological outcomes of oxidant exposure (13). This upregulation of Nrf2 through the induction of hormetic stress is foundational to the induction of an antioxidant response. Counterintuitively, the oxidative nature of ozone upregulates antioxidants through hormesis.

There are a variety of benefits of the upregulation of Nrf2 and subsequent hormetic response. 

Benefits may include:

  • A balanced inflammatory response
  • Increased antioxidant activity (14)
  • Mitochondrial health and biogenesis (production of new mitochondria) (15)
  • Cardiovascular health 
    • Endothelial health (16)
    • Heart function (17)
    • Blood pressure modulation (18)
  • Neurological health (19)

Additional Mechanisms of Ozone Therapy

There are additional mechanisms involved in the beneficial impacts of ozone therapy, including modulation of the immune system, balancing of the inflammatory response, and activation of hypoxia inducible factor-1α. 

Ozone-driven shift of cytokine activation pathways, from pro-inflammatory to anti-inflammatory immediately afterwards, also exert direct immunoregulatory effects on regulatory T lymphocytes as well as on the intestinal microbiota, which in turn can affect immune response (12).

HIF-1a
Source: By Emw - Own work, CC BY-SA 3.0,

The effectiveness of ozone therapy in vascular diseases may also be explained by the activation of another nuclear transcriptional factor, hypoxia inducible factor-1α (HIF-1a), which is also induced via moderate oxidative stress (21). As the name suggests, HIF-1a can be induced by hypoxia (lowered oxygen levels in the body). It is important to note that it is intermittent and not chronic hypoxia and HIF-1a elevations that are beneficial and this is accomplished through EBOO therapy.

Hypoxia-Inducible Factor (HIF)-1 is a dimeric protein complex that plays an integral role in the body’s response to low oxygen concentrations, or hypoxia (22). HIF-1 is also essential for immunological responses and is a crucial physiological regulator of homeostasis, vascularization, and anaerobic metabolism (23).

HIF-1a: Protection of Mitochondria

Source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859547/

HIF-1a effectively protects against overall oxidative stress and mitochondrial changes are vital in the oxidative stress response. HIF-1a is recruited to mitochondria in response to oxidative stress and protects against oxidative stress induced apoptosis (24). HIF-1α in mitochondria reduces ROS levels and reverses mitochondrial damage (25). 

Through immune regulation, a reduction in oxidative stress, and other mechanisms, HIF-1a may attenuate various pathological processes and provide multiple benefits.

These include:

  • Balanced cholesterol levels
  • Improved vascular function
  • Improved cognitive function
  • Neuroprotection
  • Balanced blood glucose levels 
  • Decreased body weight 

Through mechanisms that are often not understood or presented when discussing ozone therapy, ozone acts through multiple pathways to induce healing responses and to optimize physiological functioning. 

Ozone Therapy: Testing Outcomes

As various mechanisms of ozone therapy may reduce systemic inflammatory and oxidative stress levels and damage, testing before and during/after therapy could indicate impacts of treatment and progress.

Testing may include: 

Oxidation urinary and blood markers:

  • Urinary 8-hydroxy-2′ -deoxyguanosine (8-OHdG) 
  • Urinary lipid peroxides
  • Blood Oxidized LDL (OxLDL)
  • Blood Lipoprotein (a)

Inflammation blood markers markers:

  • C reactive protein (hs-CRP)
  • Lp-PLA2 
  • Erythrocyte sedimentation rate

Ozone Therapy: Contraindications

Although ozone therapy has proven to be extraordinarily safe for most individuals, the following conditions may be contraindicated. Please speak with an experienced ozone therapy provider before undertaking ozone therapy if you have any of the following health concerns.

  • Uncontrolled hyperthyroidism.
  • Thrombocytopenia (low platelet count).
  • Severe cardiovascular instability.
  • Seizure disorders.
  • Glucose-6-phosphate dehydrogenase deficiency.

Ultraviolet (UV) Radiation/Light

Ultraviolet radiation/light is an additional treatment modality included in 03UV therapy (the UV component of 03UV) and provides various additional benefits. Combining the positive impacts of ozone treatment with the spectrum of ultraviolet light induces a synergistic impact through their overlapping and complementary effects.

From the CDC:

Ultraviolet (UV) radiation is a form of non-ionizing radiation that is emitted by the sun and artificial sources, such as tanning beds. The beneficial effects of UV radiation include the production of a vital nutrient, vitamin D; however, overexposure may present risks. Sunburn, premature aging, and skin cancer are all risks to overexposure.

Ultraviolet light is not visible to the human eye and there are various wavelengths of UV radiation including:

  • UVA: 315 – 400 nm wavelength
  • UVB: 280 – 315 nm wavelength
  • UVC: 200 – 280 nm wavelength 

As with ozone, ultraviolet light can be both detrimental and beneficial depending on the circumstance. 

Ultraviolet Light and Environmental Sterilization

As with ozone, ultraviolet light, particularly UVC, can be used to sterilize the environment and quickly kills viruses, bacteria, yeast, and fungi. 

Uses include disinfection of: 

  • Air. 
  • Water.
  • Surfaces.
  • Foods.

In addition to its use as an environmental disinfectant, evidence suggests that direct exposure of blood to UV light can support health in a variety of ways. 

Ultraviolet Blood Irradiation (UVBI or UBI)

Ultraviolet blood irradiation (UBI) was extensively used in the 1940s and 1950s to treat many diseases including septicemia, pneumonia, tuberculosis, arthritis, asthma and even poliomyelitis (16). The early studies were carried out by several physicians in USA and published in the American Journal of Surgery (17). However with the development of antibiotics, UBI use declined and it has now been called “the cure that time forgot” (18).

UVBI as part of 03UV treatment runs the same blood being processed with ozone through a chamber exposing the blood to all three spectrums of UV light: UVA, UVB, and UVC. This provides a variety of potential benefits.

Benefits of Ultraviolet Blood Irradiation:

  • Immunomodulation (immune balancing): 
    • Upregulation of certain aspects of the immune system and downregulation of others.
    • Potential benefit in severe immune system overreaction such in cytokine storm and sepsis.
  • Potential benefit in infectious diseases.
  • Upregulation of antioxidant defenses through induction of hormetic stress.

UVBI: Mechanisms of Action

UVBI: Mechanisms of Action

UVBI functions through a variety of mechanisms. 

These include:

  • Provides a mild hormetic stress, upregulating the antioxidant response.
  • Inactivates both viruses and bacteria through inactivation of DNA and RNA (19).
  • Activates an adaptive immune response and immunomodulation through modulation of toll-like receptor function (20).
  • Upregulation of nitric oxide (NO) by photomodified neutrophils due to the activation of the iNOS enzyme (21).
  • Inhibition of lymphocytic activity, potentially reducing systemic inflammation (22).

UVBI: Contraindications

Although UVBI has proven to be extraordinarily safe for most individuals, the following conditions may be contraindicated. Please speak with an experienced UVBI therapy provider before undertaking UVBI therapy if you have any of the following health concerns.

  • Porphyria (a build up of porphyrins in the blood).

Utilizing 03UV/EBOO Treatment

There are a wide range of treatment applications for the combination of EBOO and UV treatment. It is highly beneficial for those seeking to either optimize wellness or to compliment therapies being utilized in the treatment of various health conditions. Although 03UV doesn’t treat any conditions, it is highly supportive during the process of healing from a range of disorders.

03UV: The Treatment Process

The process of being treated with 03UV is incredibly comfortable and easy. A skilled practitioner will insert an IV into each arm – one to draw blood out of the body and through the ozone and UV treatments and another to return the blood into circulation. The entire procedure takes around an hour and many people find they quickly start feeling a sense of relaxed energy as the treatment takes effect. This sense often lasts for at least a few days after the treatment.

Medicine with Heart is proud to offer this innovative treatment to both our members and also anyone who is interested in obtaining the wide variety of benefits it offers. Those who are interested will complete a simple intake to ensure it is a good fit for their circumstances. Please reach out to the clinic for more information. 

Summary

03UV is an innovative treatment combining ozone and UV therapies in order to provide a wide range of benefits. In this article, we reviewed:

  • What EBOO and 03UV are.
  • What ozone is.
  • Detrimental and therapeutic aspects of ozone.
  • What ozone therapy is.
  • Mechanisms of ozone therapy including hormetic stress.
  • What ultraviolet (UV) light is.
  • What ultraviolet light therapy (UVBI) is.
  • Mechanisms of UVBI.
  • How to utilize EBOO and 03UV in your path to optimal wellness.
  • How 03UV can support optimal wellness and compliment therapies for health concerns.
  • What it is like to receive EBOO/03/UV therapy.

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